Sancho does not want Quixote to take on this quest, because he is suspicious of duennas in general. Doña Rodríguez responds that squires in general are likewise the enemies of duennas, who are virtuous and downtrodden. Suddenly they hear a fife and drum announcing the arrival of the Dolorous Duenna herself.
Sancho’s inexplicable hatred of duennas seems to be nothing more than comic relief. It also adds to the class tensions of these episodes – especially the false friendship of the royal couple.